Establishment and Characterization of a Childhood T - Cell Acute Lymphoblastic Leukemia Cell
نویسنده
چکیده
A human leukemia cell line, PER-255, was established from the bone marrow of a 5-year-old boy with features typical of lymphomatous T-acute lymphoblastic leukemia (T-ALL). The leukemic origin of cell line PER-255 is indicated by its cytochemical and immunologic similarity to the patient’s fresh leukemic cells, which correspond to immature cortical thymocytes. Southern blot analysis showed that the lgJ genes were in germline configuration. whereas both alleles of the T-cell receptor1 (TCR-$) gene were rearranged in PER-255 cells, with identical rearrangements present in the patient’s leukemic cells. Cytogenetic analy-
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